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- Title
サラシア属植物に含有されるポリフェノール成分のLC-MS 定量分析.
- Authors
森川敏生; 赤木淳二; Pongpiriyadacha Yutana; 吉川雅之; 二宮清文; 村岡 修
- Abstract
A simultaneous quantitative analytical method for eight polyphenols, e.g. mangiferin (1), gallic acid (2), protpcatechuic acid (3), (+)-catechin (4), (-)-epicatechin (5), gallocatechin (6), (-)-epigallocatechin (7), and (-)-4'-O-methylepigallocatechin (8), as functional constituents in the roots and stems of the genus Salacia species (Hippocraceae) was developed. The content of caffeine (9), which showed similar chromatographic behavior under the analytical conditions, was also determined. The optimum conditions for separation and detection of these polyphenol constituents were achieved on an ODS column (3 μm particle size, 2.1 mm i.d. × 100 mm) with methanol / 0.1% aqueous formic acid solvent system as the mobile phase, associated with MS equipped with an electrospray ionization source. Totally 47 Salacia samples from the different origin including S. chinensis (2 roots and 28 stems), S. reticulata (6 roots and 3 stems), and S. oblonga (8 roots) were analyzed, and the results indicated that mangiferin (1) had a characteristic distributions due to the plant part [roots (1.95-40.5 mg/g from the dried material) > stems (< 0.258 mg/g)]. Among the root part, the inner root bark was found to observe the richest content of mangiferin (1).
- Publication
Japanese Journal of Food Chemistry & Safety, 2018, Vol 25, Issue 3, p130
- ISSN
1341-2094
- Publication type
Article