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- Title
用于组织工程软骨支架的改性水凝胶初步筛选.
- Authors
张姝江; 陈艺; 姚咏嫦; 谭帼馨; 施雪涛
- Abstract
Objective: To make a quick screening of the hydrogels with different compositions for cartilage tissue engineering. Methods: Compound materials of PEGD/NVP and PEGD/HEMA in two different portions (8:2 and 6:4) were co-cultured with the MSCs which were seeded onto the materials. SEM observation was performed, and the composite of the appropriate portion and component was chosen which was then treated by plasma surface modification, vacuum dehydration, or lyophilization respectively. The cytocompability of the material was evaluated together with the mechanical characteristics. Results: No cell adhesion and proliferation was observation the surface of PEGDA/HEMA(8:2) nor the surface of PEGDA/NVP(8:2) according to SEM. The cells on both PEGDA/HEMA(6:4) and PEGDA/NVP(6:4) grew and spread well, yet the extracellular matrix deposited better on the former than the latter. Thus PEGDA/HEMA (6:4) was chosen to be treated by the three different modification progresses respectively. The SEM demonstrated that the cells grew well with obvious proliferation and fusing together on the three types of modified PEGDA/HEMA (6:4); large amount of extraeellular matrix deposition could also be seen. The PEGDA/HEMA (6:4) modified by plasma showed a much better cell morphology. No significant change has been found in the compression stress of PEGDA/HEMA(6:4). Conclusions: PEGDA/HEMA(6:4) has a good eytocompatibility, and is especially good for the cell growth after plasma surface modification which makes it a potential biornaterial for the scaffold of tissue engineering cartilage.
- Publication
Progress in Modern Biomedicine, 2017, Vol 17, Issue 35, p6813
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2017.35.004