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- Title
Construction, expression, and characterization of thymosin alpha 1 tandem repeats in Escherichia coli.
- Authors
Xue, Xiao-Chang; Yan, Zhen; Li, Wei-Na; Li, Meng; Qin, Xin; Zhang, Cun; Hao, Qiang; Wang, Zeng-Lu; Zhao, Ning; Zhang, Wei; Zhang, Ying-Qi
- Abstract
Thymosin alpha 1 (T ¿ 1), which is composed of 28 amino acids, has been commercialized worldwide for its immune-modulatory and antitumor effects. T ¿ 1 can stimulate T cell proliferation and differentiation from bone marrow stem cells, augment cell-mediated immune responses, and regulate homeostasis of immune system. In this study, we developed a novel strategy to produce T ¿ 1 concatemer (T ¿ 1¿) in Escherichia coli and compared its activity with chemically synthesized T ¿ 1. Results showed that T ¿ 1¿ can more effectively stimulate T cell proliferation and significantly upregulate IL-2 receptor expression. We concluded that the expression system for T ¿ 1 concatemer was constructed successfully, which could serve as an efficient tool for the production of large quantities of the active protein.
- Publication
BioMed Research International, 2013, Vol 2013, p720285
- ISSN
2314-6133
- Publication type
Journal Article
- DOI
2013/720285