We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Are we ready to detect nematode diversity by next generation sequencing?
- Authors
Peham, Thomas; Steiner, Florian M.; Schlick-Steiner, Birgit C.; Arthofer, Wolfgang
- Abstract
In a Technical Advance article, Porazinska et al. (2009, Molecular Ecology Resources, 9, 1439-1450) assessed next generation sequencing ( NGS) as a method for metagenomic analysis of nematode diversity. We agree that NGS has great potential here. However, it is not an easy path to the successful implementation of NGS for environmental DNA analysis of nematodes. Here, we describe the method's limitations and discuss prospective research questions. For instance, only a few direct extraction kits are suitable for nematode DNA extraction from bulk samples without adaptation. They enable the analysis of extracellular nematode DNA. The most crucial and unresolved issue remains the limited availability of suitable primers.
- Subjects
NEMATODE genetics; METAGENOMICS; MOLECULAR ecology; DNA analysis; DNA primers
- Publication
Ecology & Evolution (20457758), 2017, Vol 7, Issue 12, p4147
- ISSN
2045-7758
- Publication type
Article
- DOI
10.1002/ece3.2998