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- Title
Characterization by MALDI-TOF MS and 16S rRNA Gene Sequencing of Aerobic Endospore-Forming Bacteria Isolated from Pharmaceutical Facility in Rio de Janeiro, Brazil.
- Authors
Caldeira, Nathalia Gonçalves Santos; de Souza, Maria Luiza Soares; de Miranda, Rebeca Vitória da Silva Lage; da Costa, Luciana Veloso; Forsythe, Stephen James; Zahner, Viviane; Brandão, Marcelo Luiz Lima
- Abstract
Bacillus and related genera are among the most important contaminants in the pharmaceutical production environment, and the identification of these microorganisms at the species level assists in the investigation of sources of contamination and in preventive and corrective decision making. The aim of this study was to evaluate three methodologies for the characterization of endospore-forming aerobic bacterial strains isolated from a pharmaceutical unit in Rio de Janeiro, Brazil. MALDI-TOF MS was performed using MALDI Biotyper® and VITEK® MS RUO systems, and complete 16S rRNA gene sequencing was performed using the Sanger methodology. The results showed the prevalence of the genera Bacillus (n = 9; 36.0%), Priestia (n = 5; 20.0%), and Paenibacillus (n = 4; 16.0%). Three (20.0%) strains showed <98.7% of DNA sequencing similarity on the EzBioCloud Database, indicating possible new species. In addition, the reclassification of Bacillus pseudoflexus to the genus Priestia as Priestia pseudoflexus sp. nov. is proposed. In conclusion, 16S rRNA and MALDI TOF/MS were not sufficient to identify all strains at the species level, and complementary analyses were necessary.
- Subjects
RIO de Janeiro (Brazil); SPOREFORMING bacteria; AEROBIC bacteria; RIBOSOMAL RNA; DNA sequencing; BACILLUS (Bacteria); PAENIBACILLUS
- Publication
Microorganisms, 2024, Vol 12, Issue 4, p724
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms12040724