We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.
- Authors
Drejer, Eivind B.; Hakvåg, Sigrid; Irla, Marta; Brautaset, Trygve
- Abstract
Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins. In particular, for thermostable proteins, there is a need for hosts able to properly synthesize, fold, and excrete these in high yields, and thermophilic Bacillaceae represent one potentially interesting group of microorganisms for such purposes. A number of thermophilic Bacillaceae including B.methanolicus, B.coagulans, B.smithii, B.licheniformis, Geobacillus thermoglucosidasius, G. kaustophilus, and G. stearothermophilus are investigated concerning physiology, genomics, genetic tools, and technologies, altogether paving the way for their utilization as hosts for recombinant production of thermostable and other difficult-to-express proteins. Moreover, recent successful deployments of CRISPR/Cas9 in several of these species have accelerated the progress in their metabolic engineering, which should increase their attractiveness for future industrial-scale production of proteins. This review describes the biology of thermophilic Bacillaceae and in particular focuses on genetic tools and methods enabling use of these organisms as hosts for recombinant protein production.
- Subjects
BACILLACEAE diseases; THERMOPHILIC bacteria; ESCHERICHIA coli; BACILLUS subtilis genetics; RECOMBINANT protein synthesis; HEAT stability in proteins
- Publication
Microorganisms, 2018, Vol 6, Issue 2, p42
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms6020042