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- Title
Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow.
- Authors
Terence T. W. Wong; Andy K. S. Lau; Kenneth K. Y. Ho; Matthew Y. H. Tang; Joseph D. F. Robles; Xiaoming Wei; Antony C. S. Chan; Anson H. L. Tang; Edmund Y. Lam; Kenneth K. Y. Wong; Godfrey C. F. Chan; Ho Cheung Shum; Kevin K. Tsia
- Abstract
Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity - a common predicament for advancing high-speed and high-throughput cellular imaging. We here demonstrate a new imaging approach, called asymmetric-detection time-stretch optical microscopy (ATOM), which can deliver ultrafast label-free high-contrast flow imaging with well delineated cellular morphological resolution and in-line optical image amplification to overcome the compromised imaging sensitivity at high speed. We show that ATOM can separately reveal the enhanced phase-gradient and absorption contrast in microfluidic live-cell imaging at a flow speed as high as ∼10 m/s, corresponding to an imaging throughput of ∼100,000 cells/sec. ATOM could thus be the enabling platform to meet the pressing need for intercalating optical microscopy in cellular assay, e.g. imaging flow cytometry - permitting high-throughput access to the morphological information of the individual cells simultaneously with a multitude of parameters obtained in the standard assay.
- Subjects
CELL imaging; MICROSCOPY; OPTICAL images; MICROFLUIDICS; FLOW cytometry
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep03656