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- Title
Characterization of Echinostoma cinetorchis endoribonuclease, RNase H.
- Authors
Sung-Bin Lim; Seok Ho Cha; Seung Jegal; Hojong Jun; Seo Hye Park; Bo-Young Jeon; Jhang Ho Pak; Young Yil Bakh; Tong-Soo Kim; Hyeong-Woo Lee
- Abstract
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3'-O-P bond of RNA in a DNA-RNA duplex, producing 3'-hydroxyl and 5'-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
- Subjects
PARASITES; ENDORIBONUCLEASES; OLIGONUCLEOTIDES; RNA; MESSENGER RNA
- Publication
Korean Journal of Parasitology, 2017, Vol 55, Issue 4, p451
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2017.55.4.451