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- Title
11q23.1 and 11q25-qter YACs suppress tumour growth in vivo.
- Authors
Koreth, John; Bakkenist, Christopher J; Larin, Zoia; Hunt, Nicholas CA; James, Michael R; McGee, James O'D
- Abstract
Frequent allelic deletion at chromosome 11q22-q23.1 has been described in breast cancer and a number of other malignancies, suggesting putative tumour suppressor gene(s) within the ∼8 Mb deleted region. In addition, we recently described another locus, at the 11q25-qter region, frequently deleted in breast cancer, suggesting additional tumour suppressor gene(s) in this ∼2 Mb deleted region. An 11q YAC contig was accessed and three YACs, one containing the candidate gene ATM at 11q23.1, and two contiguous YACs (overlapping for ∼400 – 600 kb) overlying most of the 11q25 deleted region, were retrofitted with a G418 resistance marker and transfected into murine A9 fibrosarcoma cells. Selected A9 transfectant clones (and control untransfected and `irrelevant' alphoid YAC transfectant A9 clones) were assayed for in vivo tumorigenicity in athymic female Balb c-nu/nu mice. All the 11q YAC transfectant clones demonstrated significant tumour suppression compared to the control untransfected and `irrelevant' YAC transfected A9 cells. These results define two discrete tumour suppressor loci on chromosome 11q by functional complementation, one to a ∼1.2 Mb region on 11q23.1 (containing the ATM locus) and another to a ∼400 – 600 kb subterminal region on 11q25-qter.
- Subjects
SUPPRESSOR cells; TUMOR growth; TUMOR suppressor genes
- Publication
Oncogene, 1999, Vol 18, Issue 5, p1157
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1202372