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- Title
Alteration of γδ T cell subsets in non‐human primates transplanted with GGTA1 gene‐deficient porcine blood vessels.
- Authors
Lee, Sujin; Chung, Yun Shin; Lee, Kyo Won; Choi, Miran; Sonn, Chung Hee; Oh, Won Jun; Hong, Hun Gi; Shim, Joohyun; Choi, Kimyung; Kim, Sung Joo; Park, Jae Berm; Kim, Tae Jin
- Abstract
Background: αGal‐deficient xenografts are protected from hyperacute rejection during xenotransplantation but are still rejected more rapidly than allografts. Despite studies showing the roles of non‐Gal antibodies and αβ T cells in xenograft rejection, the involvement of γδ T cells in xenograft rejection has been limitedly investigated. Methods: Six male cynomolgus monkeys were transplanted with porcine vessel xenografts from wild‐type (n = 3) or GGTA1 knockout (n = 3) pigs. We measured the proportions and T cell receptor (TCR) repertoires of blood γδ T cells before and after xenotransplant. Grafted porcine vessel‐infiltrating immune cells were visualized at the end of experiments. Results: Blood γδ T cells expanded and infiltrated into the graft vessel adventitia following xenotransplantation of α‐Gal‐deficient pig blood vessels. Pre‐ and post‐transplant analysis of γδ TCR repertoire revealed a transition in δ chain usage post‐transplantation, with the expansion of several clonotypes of δ1, δ3, or δ7 chains. Furthermore, the distinctions between pre‐ and post‐transplant δ chain usages were more prominent than those observed for γ chain usages. Conclusion: γδ TCR repertoire was significantly altered by xenotransplantation, suggesting the role of γδ T cells in sustained xenoreactive immune responses.
- Subjects
T cells; BLOOD vessels; T cell receptors; KRA; PRIMATES; ALLOIMMUNITY
- Publication
Xenotransplantation, 2024, Vol 31, Issue 1, p1
- ISSN
0908-665X
- Publication type
Article
- DOI
10.1111/xen.12838