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- Title
Enzymatic Preparation of l-Malate in a Reaction System with Product Separation and Enzyme Recycling.
- Authors
Li, Guosi; Zhu, Fucheng; Gu, Fangli; Yin, Xinjian; Xu, Qilin; Ma, Menghua; Zhu, Li; Lu, Baowei; Chen, Naidong
- Abstract
Reaction coupling separation systems using calcium fumarate as a substrate can break the reaction equilibrium and promote the production of l-malate. However, the low reusability and stability of fumarase limit its further application. In this study, partially purified fumarase of Thermus thermophilus (87.0 U mg−1) was immobilized within konjac-κ-carrageenan beads. An amalgamation of konjac and carrageenan gum (2%) was used to form the beads, and polyethylene polyamine (0.2%) and glutaraldehyde (0.1%) were used as the cross-linking agents. The pH and temperature profiles of free and immobilized fumarases were remarkably similar. The diffusion limit of immobilized fumarase caused a decline in the maximal velocity (Vmax), whereas the kinetic constant (Km) value increased. Optimization of the parameters for biotransformation by immobilized fumarase showed that 88.3% conversion of 200 mM calcium fumarate could be achieved at 55 °C within 8 h. The beads were stored for 30 days at 4 °C with minimal loss in activity and were reusable for up to 20 cycles with 78.1% relative activity. By recycling the reaction supernatant, a total amount of 3.98 M calcium fumarate was obtained with a conversion of 99.5%, which is the highest value ever reported.
- Subjects
THERMUS thermophilus; GLUTARALDEHYDE; EQUILIBRIUM reactions; ENZYMES; AMALGAMATION; KONJAK; CARRAGEENANS
- Publication
Catalysts (2073-4344), 2022, Vol 12, Issue 6, pN.PAG
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal12060587