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- Title
Decoding endocrine cell differentiation: insights from high‐throughput CRISPR screening in human gut organoids.
- Authors
Lin, Lin; Clevers, Hans
- Abstract
The article discusses the importance of enteroendocrine cells (EECs) in the human gut, which secrete hormones that regulate various bodily functions. The differentiation of EECs from intestinal stem cells is controlled by a network of transcription factors, with NEUROG3 being a key regulator. The study used an in vitro model of human gut organoids combined with CRISPR screening to identify genes involved in EEC differentiation. The researchers discovered that the ZNF800 gene acts as a repressor of EEC differentiation and identified downstream targets of ZNF800, including other transcription factors. The study highlights the usefulness of organoid models for understanding gut physiology and provides insights into the regulation of endocrine cell development.
- Subjects
ENTEROENDOCRINE cells; ENDOCRINE cells; GASTRIC inhibitory polypeptide; CELL differentiation; HIGH throughput screening (Drug development); ORGANOIDS; GLUCAGON-like peptide 1
- Publication
Clinical & Translational Medicine, 2024, Vol 14, Issue 1, p1
- ISSN
2001-1326
- Publication type
Article
- DOI
10.1002/ctm2.1526