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- Title
Chickpea Peptide: A Nutraceutical Molecule Corroborating Neurodegenerative and ACE-I Inhibition.
- Authors
Gupta, Neha; Quazi, Sameer; Jha, Saurabh Kumar; Siddiqi, Mohammad Khursheed; Verma, Kanika; Sharma, Swapnil; Khan, Rizwan Hassan; Bhagyawant, Sameer Suresh
- Abstract
Chickpea seeds are the source of proteins in human nutrition and attribute some nutraceutical properties. Herein, we report the effects of chickpea seed bioactive peptide on albumin, insulin, lactoglobulin and lysozyme amyloid fibril formation. Employing thioflavin T (ThT) assays and circular dichroism (CD), amyloid structural binding transition was experimented to analyze the inhibition of amyloid fibril formation. The purified active peptide with a molecular mass of 934.53 Da was evaluated in vitro for its ACE-I inhibitory, antibacterial, antifungal and antidiabetic activities. Further, in vivo animal studies were carried out in wistar rats for blood pressure lowering action. In hypertensive rats, chickpea peptide decreased 131 ± 3.57 mm of Hg for systolic blood pressure and 86 ± 1.5 mm of Hg for diastolic blood pressure after 8 h intraperitoneal administration. Additionally, the peptide suppressed the fibrillation of amyloid and destabilized the preformed mature fibrils. Data emphasize efficacy of chickpea peptide vis-a-vis ACE-Inhibitory, antibacterial, antifungal, antidiabetic and anti-amyloidogenic activities, allowing us to propose this novel peptide as a suitable candidate for nutraceutical-based drugs and seems the first kind of its nature.
- Subjects
AMYLOID; IN vitro studies; ANTIFUNGAL agents; HYPERTENSION; ALBUMINS; IN vivo studies; ANIMAL experimentation; SYSTOLIC blood pressure; HYPOGLYCEMIC agents; INTRAPERITONEAL injections; RATS; INSULIN; SEEDS; GLYCOSIDASES; GLOBULINS; PEPTIDES; ANTIBIOTICS
- Publication
Nutrients, 2022, Vol 14, Issue 22, p4824
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14224824