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- Title
The Effect of Leucine-Enriched Essential Amino Acid Supplementation on Anabolic and Catabolic Signaling in Human Skeletal Muscle after Acute Resistance Exercise: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Comparison Trial.
- Authors
Takegaki, Junya; Sase, Kohei; Yasuda, Jun; Shindo, Daichi; Kato, Hiroyuki; Toyoda, Sakiko; Yamada, Toshiyuki; Shinohara, Yasushi; Fujita, Satoshi
- Abstract
Resistance exercise transiently activates anabolic and catabolic systems in skeletal muscle. Leucine-enriched essential amino acids (LEAAs) are reported to stimulate the muscle anabolic response at a lower dose than whey protein. However, little is known regarding the effect of LEAA supplementation on the resistance exercise-induced responses of the anabolic and catabolic systems. Here, we conducted a randomized, double-blind, placebo-controlled, parallel-group comparison trial to investigate the effect of LEAA supplementation on mechanistic target of rapamycin complex 1 (mTORC1), the ubiquitin–proteasome system and inflammatory cytokines after a single bout of resistance exercise in young men. A total of 20 healthy young male subjects were supplemented with either 5 g of LEAA or placebo, and then they performed 10 reps in three sets of leg extensions and leg curls (70% one-repetition maximum). LEAA supplementation augmented the phosphorylation of mTORSer2448 (+77.1%, p < 0.05), p70S6KThr389 (+1067.4%, p < 0.05), rpS6Ser240/244 (+171.3%, p < 0.05) and 4EBP1Thr37/46 (+33.4%, p < 0.05) after resistance exercise. However, LEAA supplementation did not change the response of the ubiquitinated proteins, MuRF-1 and Atrogin-1 expression. Additionally, the mRNA expression of IL-1β and IL-6 did not change. These data indicated that LEAA supplementation augments the effect of resistance exercise by enhancing mTORC1 signal activation after exercise.
- Subjects
ANALYSIS of covariance; CELLULAR signal transduction; COMPARATIVE studies; CYTOKINES; DIETARY supplements; EXERCISE physiology; LEUCINE; METABOLISM; PHOSPHORYLATION; PLACEBOS; PROTEIN kinases; PROTEOLYTIC enzymes; T-test (Statistics); WESTERN immunoblotting; RANDOMIZED controlled trials; BLIND experiment; SKELETAL muscle; SIGNAL peptides; DESCRIPTIVE statistics; RIBOSOMAL proteins; ESSENTIAL amino acids; RESISTANCE training
- Publication
Nutrients, 2020, Vol 12, Issue 8, p2421
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu12082421