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- Title
Polydatin Inhibits NLRP3 Inflammasome in Dry Eye Disease by Attenuating Oxidative Stress and Inhibiting the NF-κB Pathway.
- Authors
Park, Bongkyun; Jo, Kyuhyung; Lee, Tae Gu; Hyun, Soo-Wang; Kim, Jin Sook; Kim, Chan-Sik
- Abstract
Polydatin (also named pieceid, (E)-piceid, (E)-polydatin, trans-polydatin, or 3,5,4'-trihydroxystilbene-3-b-D-glucoside) is a monocrystalline compound isolated from the root and rhizome of Polygonum cuspidatum Sieb. et Zucc. (Polygonaceae). A previous study showed that polydatin has antioxidant and anti-inflammatory effects. However, the effect of polydatin in dry eye disease (DED) has not been elucidated. DED rat models were induced by exorbital lacrimal gland-excision. In vivo, the present study showed that the excision of lacrimal glands induced changes such as reduced tear fluid, severe corneal irregularity, damage, tear film break, and goblet cell loss as well as increased inflammation cytokine and NLRP3 expression in conjunctival tissue. However, these changes were restored by polydatin eye dropping. In vitro, polydatin inhibited hyperosmolar stress-induced inflammation through attenuation of the translocation of NF-κB to the nucleus and the mRNA expression of TNF-α, IL-6, IL-1β, and MMP9. In addition, the hyperosmolar stress-induced NLRP3 inflammasome pathway and ROS production were inhibited by polydatin. Our findings provided insight into the effect of polydatin as a candidate reagent for the treatment of DED.
- Subjects
ANTI-inflammatory agents; REACTIVE oxygen species; ANIMAL experimentation; CELLULAR signal transduction; DRY eye syndromes; GENE expression; GLYCOSIDES; INFLAMMATION; INFLAMMATORY mediators; INTERLEUKINS; MEDICINAL plants; MESSENGER RNA; MOLECULAR structure; RATS; PLANT roots; STILBENE; TUMOR necrosis factors; DNA-binding proteins; OXIDATIVE stress; IN vitro studies; IN vivo studies; PHARMACODYNAMICS
- Publication
Nutrients, 2019, Vol 11, Issue 11, p2792
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu11112792