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- Title
Imaging cellular forces with photonic crystals.
- Authors
Li, Qiwei; Chen, Zaozao; Zhang, Ying; Ding, Shuang; Ding, Haibo; Wang, Luping; Xie, Zhuoying; Fu, Yifu; Wei, Mengxiao; Liu, Shengnan; Chen, Jialun; Wang, Xuan; Gu, Zhongze
- Abstract
Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vertical cell forces over a wide field of view (1.3 mm ⨯ 1.0 mm, a 10 ⨯ objective image) at high speed (about 20 frames per second) without references. The photonic crystal hydrogel substrate (PCS) converts micro-nano deformations into perceivable color changes, enabling in situ visualization and quantification of tiny vertical cell forces with high throughput. It enabled long-term, cross-scale monitoring from subcellular focal adhesions to tissue-level cell sheets and aggregates. Current techniques for visualizing cell generated forces suffer from throughput limitations. Here, Gu et al. introduced photonic crystal cellular force microscopy, inspired by chameleons, enabling visualization and quantification of vertically directed cell forces, well-suited for drug screening.
- Subjects
PHOTONIC crystals; CELL imaging; FOCAL adhesions; DATA visualization; CHAMELEONS
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-43090-9