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- Title
Inhibitory effect of low-level laser irradiation on LPS-stimulated prostaglandin E<sub>2</sub> production and cyclooxygenase-2 in human gingival fibroblasts.
- Authors
Sakurai, Y.; Yamaguchi, M.; Abiko, Y.
- Abstract
It has been reported that lipopolysaccharide (LPS) from periodontal pathogens can penetrate gingival tissues and stimulate the production of prostaglandin E&sub2; (PGE&sub2;), which is known as a potent stimulator of inflammation and bone resorption. Although biostimulatory effects of low-level laser irradiation such as anti-inflammatory results have been reported, the physiological mechanism is not yet clarified. The purpose of the present study was to determine the effect of laser irradiation on PGE&sub2; production and cyclooxygenase (COX)-1 and COX-2 gene expression in LPS-challenged human gingival fibroblast (hGF) cells in vitro. hGF cells were prepared from healthy gingival tissues and challenged with LPS, and Ga-Al-As diode laser was irradiated to the hGF cells. The amount of PGE&sub2; released in the culture medium was measured by radioimmunoassay, and mRNAlevels were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). Irradiation with Ga-Al-As diode low-level laser significantly inhibited PGE&sub2; production in a dose-dependent manner, which led to a reduction of COX-2 mRNA levels. In conclusion, low-level laser irradiation inhibited PGE&sub2; by LPS in hGF cells through a reduction of COX-2 mRNA level. The findings suggest that low-level laser irradiation may be of therapeutic benefit against the aggravation of gingivitis and periodontitis by bacterial infection.
- Subjects
IRRADIATION; PROSTAGLANDINS; CYCLOOXYGENASE 2; FIBROBLASTS
- Publication
European Journal of Oral Sciences, 2000, Vol 108, Issue 1, p29
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1034/j.1600-0722.2000.00783.x