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- Title
The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice.
- Authors
Torbati, Elham; Bandehpour, Mojgan; Pakzad, Parviz; Mosaffa, Nariman; Koochaki, Ameneh; Kazemi, Bahram
- Abstract
Background: Hepatitis infection represents one of the important causes of morbidity and mortality in developing countries, however there is not any effective vaccine against hepatitis C which is one of the significant problems in vaccine project. Objectives: The aim of the present study is to evaluate the role of HCV core protein in inducing IFN-Gamma secretion and TCL activities as a vaccine in Balb/C mice. Materials and Methods: Our previous cloned plasmid (HCV Core gene into pETDuet-1) applied for protein expression in bacteria. The expressed and purified recombinant protein together with Freund's adjuvant was injected to 15 Balb/c mice. The total IgG and IgG2a of immunized mice sera were evaluated after a week. Two weeks after booster injection, we studied the proliferation and IFNγ secretion of spleens, inguinal and popliteal lymph nodes lymphocytes by ELISA and ELISPOT. Results: The FSFC (Frequency of spot forming cells) of secreting cells of immunized mice with HCV/Core protein and sera IgG2a were considerably higher than the control groups. Conclusions: The core protein together with proper adjuvant can be a candidate vaccine against of HCV infection.
- Subjects
IRAN; ANALYSIS of variance; ANIMAL experimentation; ANTIGENS; ENZYME-linked immunosorbent assay; GENE expression; HEPATITIS C; MICE; RECOMBINANT proteins
- Publication
Hepatitis Monthly, 2012, Vol 12, Issue 6, p391
- ISSN
1735-143X
- Publication type
Article
- DOI
10.5812/hepatmon.6141