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- Title
Sequenced RAPD markers to detect hybridization in the barbary partridge ( Alectoris barbara, Phasianidae).
- Authors
BARBANERA, FILIPPO; GUERRINI, MONICA; BERTONCINI, FRANCO; CAPPELLI, FABIO; MUZZEDDU, MARCO; DINI, FERNANDO
- Abstract
In the Alectoris partridges (Phasianidae), hybridization occurs occasionally as a result of the natural breakdown of isolating mechanisms but more frequently as a result of human activity. No genetic record of hybridization is known for the barbary partridge ( A. barbara). This species is distributed mostly in North Africa and, in Europe, on the island of Sardinia (Italy) and on Gibraltar. The risk of hybridization between barbary and red-legged partridge ( A. rufa: Iberian Peninsula, France, Italy) is high in Sardinia and in Spain. We developed two random amplified polymorphic DNA (RAPD) markers to detect A. barbara × A. rufa hybrid partridges. We tested them on 125 experimental hybrids, sequenced the relative species-specific bands and found that the bands and their corresponding sequences were reliably transmitted through a number of generations (F1, F2, F3, BC1, BC2). Our markers represent a highly valuable tool for the preservation of the A. barbara genome from the pressing threat of A. rufa pollution.
- Subjects
ALECTORIS; RED-legged partridge; SPECIES hybridization; PHASIANIDAE; GENOMES
- Publication
Molecular Ecology Resources, 2011, Vol 11, Issue 1, p180
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1755-0998.2010.02880.x