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- Title
Analysis of Hepatitis B virus (HBV) mutations in patients from Western Saudi Arabia with chronic disease.
- Authors
El-Kafrawy, Sherif Aly; Jamjoom, Ghazi Abdulatif; Akbar, Hisham Othman; Fallatah, Hind Ibrahim Baker; El-Daly, Mai Mohamed; Qari, Yousef Abdulfattah; Alghamdi, Abdullah Saeed; Babatin, Mohamed; Alsaedi, Mohammed Abdullah; Othman, Norah Abdulhamid; Al-Subhi, Tagreed Lafi; Abdel-Hamid, Mohamed; Azhar, Esam Ibraheem
- Abstract
Introduction: Extensive research has provided a link between HBV variants and the clinical complications of liver diseases. This study was performed to further investigate the relationship between HBV variants in preS, S and BCP/PC regions and disease progression in chronic hepatitis B (CHB) cases in Jeddah, Saudi Arabia. Methodology: 182 CHB patients were recruited for this study. HBV DNA was amplified by PCR in the PreS, S, and BCP/PC regions. Sequences were generated from 31 and 26 treated cases in PreS and S regions respectively and from 72 cases in the BCP/PC region. Results: The majority of cases (86.7%) were genotype D. Mutations at preS1-A2922C, X-A1624C and PC-G1887A were detected only in cases with either a high fibrosis score or hepatocellular carcinoma (HCC), while mutations at positions PC-C1982A, PC-G1951T, X-C1628T and XA1630G were detected more frequently in HCC cases, without reaching statistical significance. Seven deletions were detected in the PreSregion. No deletions were detected in the CCAAT box. The accumulation of mutations per sample in the preS1-2 and S regions were associated with elevated ALT (p < 0.001, 0.001 and 0.001; respectively) and increased fibrosis (p = 0.018, 0.02 and 0.013; respectively). The accumulation of mutations per sample in the BCP/PC region is associated with high viral load. Occult hepatitis B infection (OBI) was identified in 5 samples. Conclusion: Our results add to the knowledge about HBV genotype-D variants. The accumulation of mutations per sample and OBI seem to play a role in the progression of HBV infection. G1896A was associated with the HBeAg negativity. The preS deletions did not play a role in liver disease progression.
- Subjects
HEPATITIS B virus; GENETIC mutation; LIVER diseases; CHRONIC hepatitis B; DISEASE complications; DISEASE progression
- Publication
Journal of Infection in Developing Countries, 2018, Vol 12, Issue 7, p557
- ISSN
2036-6590
- Publication type
Article
- DOI
10.3855/jidc.9534