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- Title
Simultaneous determination of four bioactive compounds in Verbena officinalis L. by using high-performance liquid chromatography.
- Authors
Zhen Liu; Zhiwei Xu; Hanyu Zhou; Gang Cao; Xiao-Dong Cong; Yun Zhang; Bao-Chang Cai
- Abstract
Background: Verbena officinalis L., called mabiancao in Chinese, is derived from the aerial part of Herba Verbanae. It is a traditional Chinese medicine commonly used in China and northern Europe, which is widely used for clearing away heat and detoxicating, promoting blood circulation, and removing blood stasis. This paper describes a sensitive and specific assay for the determination of four bioactive compounds in V. officinalis L. Materials and Methods: In this paper, the four components were separated on an Agilent Zorbax Extend C18 column (250 mm x 4.6 mm x 5 μm) and detected by a diode array detector. The mobile phase was composed of (a) aqueous phosphoric acid (0.1%, v/v) and (b) acetonitrile using a gradient elution. Analytes were performed at 30ºC with a flow rate of 1.0 ml/min and UV detection at 203, 238, and 331 nm. Results: All calibration curves showed good linear regression (r2 ≥ 0.9999) within tested ranges. Overall intraand interday variations were less than 1.84%, and the average recoveries were 97.32-102.81% for analytes. Discussion and Conclusion: The proposed method would be sensitive enough and reliable for comprehensive quality control for clinical use and modernization of V. officinalis L.
- Subjects
VERBENA; LIQUID chromatography; BIOACTIVE compounds; PLANT products; PHYTOTHERAPY; MEDICINAL plants; MEDICAL botany; THERAPEUTICS
- Publication
Pharmacognosy Magazine, 2012, Vol 8, Issue 30, p162
- ISSN
0973-1296
- Publication type
Article
- DOI
10.4103/0973-1296.96575