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- Title
Cluster-forming property correlated with hemolytic activity by staphylococcal γ-hemolysin transmembrane pores
- Authors
Tomita, Noriko; Abe, Kazuyo; Kamio, Yoshiyuki; Ohta, Makoto
- Abstract
Abstract: Staphylococcal γ-hemolysin (Hlg) is a pore-forming toxin consisting of two separate components, LukF (34kDa) and Hlg2 (32kDa). Here we show that Hlg pores aggregate and form clusters on human erythrocyte membranes in association with increasing hemolytic activity. Quantitative analysis using transmission electron microscopy and image processing revealed that the formation of single pores and clusters is related to the release of potassium ions and of hemoglobin from erythrocytes, respectively. This is the first study to suggest a novel and unique property which can facilitate hemolysis by the cluster formation of Hlg pores.
- Subjects
ERYTHROCYTE membranes; STAPHYLOCOCCAL diseases; TRANSMISSION electron microscopy; HEMOLYSIS &; hemolysins; STATISTICAL correlation; TOXINS; QUANTITATIVE research
- Publication
FEBS Letters, 2011, Vol 585, Issue 21, p3452
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.09.041