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- Title
Calmodulin activation of polo-like kinase 1 is required during mitotic entry.
- Authors
Gu Dai; Yan Qian; Jie Chen; Fan-Li Meng; Fei-Yan Pan; Wei-Gan Shen; Sheng-Zhou Zhang; Bin Xue; Chao-Jun Li
- Abstract
Polo-like kinase 1 (Plk1) is a conserved key regulator of the G2/M transition, but its upstream spatiotemporal regulators remain unknown. With the help of immunofluorescence, co-immunoprecipitation, and glutathione S-transferase (GST) pull-down assay, we found that calmodulin (CaM) is one such regulatory molecule that associates with Plk1 from G2 to metaphase. More importantly, this interaction results in considerable stimulation of Plk1 kinase activity leading to hyperphosphorylation of Cdc25C. Our results provide new insight into the role of CaM as an upstream regulator of Plk1 activation during mitotic entry.
- Publication
Biochemistry & Cell Biology, 2013, Vol 91, Issue 5, p287
- ISSN
0829-8211
- Publication type
Article
- DOI
10.1139/bcb-2013-0015