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- Title
Kinetics of Archived M184V Mutation in Treatment-Experienced Virally Suppressed HIV-Infected Patients.
- Authors
Palich, Romain; Teyssou, Elisa; Sayon, Sophie; Abdi, Basma; Soulie, Cathia; Cuzin, Lise; Tubiana, Roland; Valantin, Marc-Antoine; Schneider, Luminita; Seang, Sophie; Wirden, Marc; Pourcher, Valérie; Katlama, Christine; Calvez, Vincent; Marcelin, Anne-Geneviève
- Abstract
<bold>Background: </bold>We aimed to assess the kinetics of drug-resistant viral variants (DRVs) harboring the M184V mutation in proviral DNA of long-term virally suppressed patients, and factors associated with DRV persistence.<bold>Methods: </bold>Human immunodeficiency virus (HIV) DNA from blood cells stored in 2016 and 2019 was sequenced using Sanger and ultradeep sequencing (SS and UDS; detection threshold 1%) in antiretroviral therapy (ART)-treated patients with HIV RNA < 50 copies/mL for at least 5 years, with past M184V mutation documented in HIV RNA.<bold>Results: </bold>Among 79 patients, by combining SS and UDS, M184V was found to be absent in 26/79 (33%) patients and persistent in 53/79 (67%). M184V-positive patients had a longer history of ART, lower CD4 nadir, and higher pretherapeutic HIV RNA. Among 37 patients with viral sequences assessed by UDS, the proportion of M184V-positive DRVs significantly decreased between 2016 and 2019 (40% vs 14%, P = .005). The persistence of M184V was associated with duration and level of HIV RNA replication under lamivudine/emtricitabine (3TC/FTC; P = .0009 and P = .009, respectively).<bold>Conclusions: </bold>While it decreased over time in HIV DNA, M184V mutation was more frequently persistent in HIV DNA of more treatment-experienced patients with longer past replication under 3TC/FTC.
- Subjects
HIV; HIV-positive persons; BLOOD banks; CHRONIC hepatitis B; GENETIC mutation; ANTIRETROVIRAL agents
- Publication
Journal of Infectious Diseases, 2022, Vol 225, Issue 3, p502
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiab413