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- Title
The effect of magnesium and vitamin E pre-treatments on irradiationinduced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental study.
- Authors
Atasoy, Beste M.; Özgen, Zerrin; Bostanci, Korkut; Yüksel, Meral; Özen, Zeynep; İbrahimov, Roman; Abacioğ;lu, Ufuk
- Abstract
Objective: The aim of this study was to investigate the effect of pre-treatment with the free radical scavenging molecules, magnesium and vitamin E, on lipid peroxidation to limit radiation-induced heart and lung injury. Methods: Female Sprague-Dawley rats were divided into 4 groups by a simple randomization method as saline-treated control (n=4), salinetreated irradiated (IR; n=6), magnesium sulphate-treated irradiation (IR) (Mg+IR; n=6) and vitamin E-treated IR (vit E+IR; n=6), respectively. The animals were given either saline, Mg (600mg/kg/day) or vit E (100 mg/kg/day) intraperitoneally for five days prior to irradiation. Twelve hours after the fifth injection, animals in irradiation groups were irradiated to 20 Gy using 6 MV photons in linear accelerator. Twenty-four hours later cardiac and lung tissue samples were obtained for determination of myeloperoxidase activity (MPO), malondialdehyde (MDA) levels, and luminol and lucigenin levels measured by chemiluminescence (CL) methods. Results: No significant changes were observed between cardiac and pulmonary MDA and CL results of the experimental groups. However, cardiac and pulmonary MPO activities in the saline-treated IR group were increased as compared to control group (p<0.05 for all), while in the Mg-pretreated and vit E pretreated groups neutrophil infiltration was reduced, reaching to statistical significance only in the Mg-pretreated group (p<0.05). Conclusion: Prophylactic use of magnesium sulfate has limited the infiltration of neutrophils to both the cardiac and pulmonary tissues at the early 24 h of irradiation. However, how limiting neutrophils as the sources of free radicals and inflammatory mediators would alter oxidative stress of heart and lung tissues in the long-term is not clear yet. (Anadolu Kardiyol Derg 2012; 12: 508-14)
- Subjects
MAGNESIUM; VITAMIN E; IRRADIATION; PULMONARY artery; TISSUE wounds; CHEMILUMINESCENCE; WOUNDS &; injuries
- Publication
Anatolian Journal of Cardiology / Anadolu Kardiyoloji Dergisi, 2012, Vol 12, Issue 6, p508
- ISSN
2149-2263
- Publication type
Article
- DOI
10.5152/akd.2012.159