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- Title
Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity.
- Authors
Degen, Joachim; Dublin, Pavel; Zhang, Jiong; Dobrowolski, Radoslaw; Jokwitz, Melanie; Karram, Khalad; Trotter, Jacqueline; Jabs, Ronald; Willecke, Klaus; Steinhäuser, Christian; Theis, Martin
- Abstract
Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas.
- Subjects
ASTROCYTES; GENE silencing; CONNEXINS; GAP junctions (Cell biology); GLIAL fibrillary acidic protein
- Publication
FASEB Journal, 2012, Vol 26, Issue 11, p4576
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.12-207183