We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Neuropeptide Y inhibits interleukin-1 beta-induced microglia motility.
- Authors
Ferreira, Raquel; Santos, Tiago; Cortes, Luísa; Cochaud, Stéphanie; Agasse, Fabienne; Silva, Ana Paula; Xapelli, Sara; Malva, João O.
- Abstract
J. Neurochem. (2012) 120, 93-105. Abstract Increasing evidences suggest that neuropeptide Y (NPY) may act as a key modulator of the cross-talk between the brain and the immune system in health and disease. In the present study, we dissected the possible inhibitory role of NPY upon inflammation-associated microglial cell motility. NPY, through activation of Y1 receptors, was found to inhibit lipopolysaccharide (LPS)-induced microglia (N9 cell line) motility. Moreover, stimulation of microglia with LPS was inhibited by IL-1 receptor antagonist (IL-1ra), suggesting the involvement of endogenous interleukin-1 beta (IL-1β) in this process. Direct stimulation with IL-1β promoted downstream p38 mitogen-activated protein kinase mobilization and increased microglia motility. Moreover, consistently, p38 mitogen-activated protein kinase inhibition decreased the extent of actin filament reorganization occurring during plasma membrane ruffling and p38 phosphorylation was inhibited by NPY, involving Y1 receptors. Significantly, the key inhibitory role of NPY on LPS-induced motility of CD11b-positive cells was further confirmed in mouse brain cortex explants. In summary, we revealed a novel functional role for NPY in the regulation of microglial function that may have important implications in the modulation of CNS injuries/diseases where microglia migration/motility might play a role.
- Subjects
NEUROPEPTIDE Y; INTERLEUKIN-1; MICROGLIA; LIPOPOLYSACCHARIDES; INFLAMMATION
- Publication
Journal of Neurochemistry, 2012, Vol 120, Issue 1, p93
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2011.07541.x