We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Endogenous purinergic signaling is required for osmotic volume regulation of retinal glial cells.
- Authors
Wurm, Antje; Lipp, Stephan; Pannicke, Thomas; Linnertz, Regina; Krügel, Ute; Schulz, Angela; Färber, Katrin; Zahn, Dirk; Grosse, Johannes; Wiedemann, Peter; Ju Chen; Schöneberg, Torsten; Illes, Peter; Reichenbach, Andreas; Bringmann, Andreas
- Abstract
J. Neurochem. (2010) 112, 1261–1272. Intense neuronal activity in the sensory retina is associated with a volume increase of neuronal cells (Uckermann et al., J. Neurosci. 2004, 24:10149) and a decrease in the osmolarity of the extracellular space fluid (Dmitriev et al., Vis. Neurosci. 1999, 16:1157). Here, we show the existence of an endogenous purinergic mechanism that prevents hypoosmotic swelling of retinal glial (Müller) cells in mice. In contrast to the cells from wild-type mice, hypoosmotic stress induced rapid swelling of glial cell somata in retinal slices from mice deficient in P2Y1, adenosine A1 receptors, or ecto-5′-nucleotidase (CD73). Consistently, glial cell bodies in retinal slices from wild-type mice displayed osmotic swelling when P2Y1 or A1 receptors, or CD73, were pharmacologically blocked. Exogenous ATP, UTP, and UDP inhibited glial swelling in retinal slices, while the swelling of isolated glial cells was prevented by ATP but not by UTP or UDP, suggesting that uracil nucleotides indirectly regulate the glial cell volume via activation of neuronal P2Y4/6 and neuron-to-glia signaling. It is suggested that autocrine/paracrine activation of purinergic receptors and enzymes is crucially involved in the regulation of the glial cell volume.
- Subjects
NEUROGLIA; PURINERGIC receptors; RETINAL ganglion cells; OSMOLAR concentration; EDEMA; NUCLEOTIDES; THERAPEUTICS
- Publication
Journal of Neurochemistry, 2010, Vol 112, Issue 5, p1261
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2009.06541.x