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- Title
CCNB1 affects cavernous sinus invasion in pituitary adenomas through the epithelial-mesenchymal transition.
- Authors
Li, Bin; Cheng, Jianhua; Wang, Hongyun; Zhao, Sida; Zhu, Haibo; Li, Chuzhong; Zhang, Yazhuo; Zhao, Peng
- Abstract
<bold>Background: </bold>To investigate the relationship between cyclin B1 (CCNB1) gene expression and cavernous sinus invasion in pituitary adenomas.<bold>Methods: </bold>Twenty-four pituitary adenoma tissue samples were examined by RT-qPCR and Western blot to assess the mRNA expression levels and protein levels of CCNB1, E-cadherin and N-cadherin. Correlation analyses between the expression levels of E-cadherin, N-cadherin and CCNB1 were performed. After lentivirus-mediated knockdown of CCNB1 in rat pituitary adenoma cell lines (GH3 and GT1-1), cell function changes were studied. The relationship between CCNB1 and epithelial-mesenchymal transition (EMT) was further verified by animal experiments.<bold>Results: </bold>CCNB1 and N-cadherin gene expression were significantly higher in the invasive pituitary adenomas than in the non-invasive pituitary adenomas. Conversely, E-cadherin expression in the invasive pituitary adenomas was significantly lower. CCNB1 gene expression was downregulated in the GH3 and GT1-1 pituitary adenoma cell lines; N-cadherin expression was also decreased, but E-cadherin expression was increased. These results were confirmed in vivo. After downregulation of CCNB1, cell invasion and migration was significantly reduced in Transwell experiments.<bold>Conclusion: </bold>High CCNB1 expression in pituitary adenoma affects cavernous sinus invasion through EMT.
- Subjects
CAVERNOUS sinus; PROTEIN expression; PITUITARY gland; GENE expression; CELL physiology; SPHENOID sinus; ADENOMATOUS polyps; CANCER invasiveness
- Publication
Journal of Translational Medicine, 2019, Vol 17, Issue 1, pN.PAG
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-019-2088-8