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- Title
Bee venom ameliorates lipopolysaccharide-induced memory loss by preventing NF-kappaB pathway.
- Authors
Sun Mi Gu; Mi Hee Park; Chul Ju Hwang; Ho Sueb Song; Ung Soo Lee; Sang Bae Han; Ki Wan Oh; Young Wan Ham; Min Jong Song; Dong Ju Son; Jin Tae Hong
- Abstract
Background: Accumulation of beta-amyloid and neuroinflammation trigger Alzheimer's disease. We previously found that lipopolysaccharide (LPS) caused neuroinflammation with concomitant accumulation of beta-amyloid peptides leading to memory loss. A variety of anti-inflammatory compounds inhibiting nuclear factor kappaB (NF-κB) activation have showed efficacy to hinder neuroinflammation and amyloidogenesis. We also found that bee venom (BV) inhibits NF-κB. Methods: A mouse model of LPS-induced memory loss used administration of BV (0.8 and 1.6 µg/kg/day, i.p.) to ICR mice for 7 days before injection of LPS (2.5 mg/kg/day, i.p.). Memory loss was assessed using a Morris water maze test and passive avoidance test. For invitro study, we treated BV (0.5, 1, and 2 µg/mL) to astrocytes and microglial BV-2 cells with LPS (1 µg/mL). Results: We found that BV inhibited LPS-induced memory loss determined by behavioral tests as well as cell death. BV also inhibited LPS-induced increases in the level of beta-amyloid (Aβ), β-and γ-secretases activities, NF-κB and its DNA-binding activity and expression of APP, and BACE1 and neuroinflammation proteins (COX-2, iNOS, GFAP and IBA-1) in the brain and cultured cells. In addition, pull-down assay and molecular modeling showed that BV binds to NF-κB. Conclusions: BV attenuates LPS-induced amyloidogenesis, neuroinflammation, and therefore memory loss via inhibiting NF-κB signaling pathway. Thus, BV could be useful for treatment of Alzheimer's disease.
- Subjects
BEE venom; MEMORY loss; NF-kappa B; AMYLOID beta-protein; PHYSIOLOGICAL effects of lipopolysaccharides; NEUROLOGICAL disorders; PHYSIOLOGY; THERAPEUTIC use of venom
- Publication
Journal of Neuroinflammation, 2015, Vol 12, Issue 1, p1
- ISSN
1742-2094
- Publication type
Article
- DOI
10.1186/s12974-015-0344-2