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- Title
Single-walled carbon nanotube interactions with HeLa cells.
- Authors
Yehia, Hadi N.; Draper, Rockford K.; Mikoryak, Carole; Walker, Erin Kate; Bajaj, Pooja; Musselman, Inga H.; Daigrepont, Meredith C.; Dieckmann, Gregg R.; Pantano, Paul
- Abstract
This work concerns exposing cultured human epithelial-like HeLa cells to single-walled carbon nanotubes (SWNTs) dispersed in cell culture media supplemented with serum. First, the as received CoMoCAT SWNT-containing powder was characterized using scanning electron microscopy and thermal gravimetric analyses. Characterizations of the purified dispersions, termed DM-SWNTs, involved atomic force microscopy, inductively coupled plasma -- mass spectrometry, and absorption and Raman spectroscopies. Confocal microRaman spectroscopy was used to demonstrate that DM-SWNTs were taken up by HeLa cells in a time- and temperature-dependent fashion. Transmission electron microscopy revealed SWNT-like material in intracellular vacuoles. The morphologies and growth rates of HeLa cells exposed to DM-SWNTs were statistically similar to control cells over the course of 4 d. Finally, flow cytometry was used to show that the fluorescence from MitoSOX™ Red, a selective indicator of superoxide in mitochondria, was statistically similar in both control cells and cells incubated in DM-SWNTs. The combined results indicate that under our sample preparation protocols and assay conditions, CoMoCAT DM-SWNT dispersions are not inherently cytotoxic to HeLa cells. We conclude with recommendations for improving the accuracy and comparability of carbon nanotube (CNT) cytotoxicity reports.
- Subjects
HELA cells; CARBON nanotubes; CELL culture; SERUM; SCANNING electron microscopy; THERMOGRAVIMETRY; ATOMIC force microscopy
- Publication
Journal of Nanobiotechnology, 2007, Vol 5, p8
- ISSN
1477-3155
- Publication type
Article
- DOI
10.1186/1477-3155-5-8