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- Title
PGM5 is a promising biomarker and may predict the prognosis of colorectal cancer patients.
- Authors
Sun, Yifan; Long, Haihua; Sun, Lin; Sun, Xiujuan; Pang, Liping; Chen, Jianlin; Yi, Qingqun; Liang, Tianwei; Shen, Yongqi
- Abstract
Background: Phosphoglucomutase (PGM), a key enzyme in the metabolism of glucose-1-phosphate and glucose-6-phosphate, has been found to be associated with proliferation, invasion, and metastasis of cancer. However, the expression and function of PGM5 in colorectal cancer (CRC) remains unknown. Methods: We tested PGM5 mRNA and protein expression levels in 79 CRC tissue and their matched adjacent tissue samples by qRT-PCR and immunohistochemistry, respectively. Overall survival (OS) was estimated with the Kaplan–Meier method and compared between groups with the log-rank test. We performed multivariable Cox regression analyses to identify factors associated with CRC risk. The cell proliferation, migration and invasion abilities of CRC cells were detected by using CCK-8, Transwell migration and invasion assays, respectively. Results: The PGM5 protein levels expression in CRC tissues were significantly lower than those in the adjacent tissues (t = 5.035, P < 0.001), and Kaplan–Meier analysis indicated that low PGM5 expression were significantly associated with poor overall survival (P = 0.0069). Univariate and multivariate analyses demonstrated that PGM5 was an independent risk factor for overall survival (hazard ratio = 0.3951, P = 0.014). PGM5 overexpression significantly inhibited the proliferation, invasion and migration abilities of CRC cells. On the contrary, knockdown of PGM5 promotes the invasion and migration of CRC cells. Conclusions: PMG5 regulates proliferation, invasion, and migration in the CRC and decreased PGM5 is associated with poor prognosis. Therefore, PGM5 is a promising biomarker in CRC and decreased PGM5 may predict poor overall survival in patients with CRC.
- Subjects
COLORECTAL cancer; CANCER prognosis; ENZYME metabolism; CANCER patients; PROTEIN expression
- Publication
Cancer Cell International, 2019, Vol 19, Issue 1, pN.PAG
- ISSN
1475-2867
- Publication type
Article
- DOI
10.1186/s12935-019-0967-y