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- Title
Discrepancies between VEGF-1154 G>A Polymorphism Analysis Performed in Peripheral Blood Samples and FFPE Tissue.
- Authors
Marisi, Giorgia; Passardi, Alessandro; Calistri, Daniele; Zoli, Wainer; Amadori, Dino; Ulivi, Paola
- Abstract
Single nucleotide polymorphisms (SNPs) may be associated with the response or toxicity to different types of treatment. Although SNP analysis is usually performed on DNA from peripheral blood, formalin fixed paraffin-embedded (FFPE) tissue is often used for retrospective studies. We analyzed VEGF (-2578C>A, -1498C>T, -1154G>A, -634C>G, +936C>T) and eNOS (+894G>T, -786T>C, VNTR (variable number of tandem repeats) 27bp intron 4) polymorphisms by direct sequencing or Real Time PCR in 237 patients with advanced colorectal cancer. Peripheral blood was used for 153 patients, whereas only FFPE tumor tissue was available for 84 patients. All SNP frequencies were in Hardy-Weinberg Equilibrium (HWE), with the exception of VEGF -1154, which was only in HWE in peripheral blood specimens. We therefore analyzed this SNP in DNA extracted from FFPE tumor tissue compared to FFPE healthy tissue and peripheral blood from 20 patients. Numerous heterozygous patients in peripheral blood DNA were homozygous for the A-allele in both tumor and healthy FFPE tissues. Our findings indicate that, although FFPE tissue might be a suitable specimen for genotyping, VEGF -1154 does not give reliable results on this type of material. As other SNPs may also have this limitation, genotype concordance should first be confirmed by comparing results obtained from FFPE and fresh sample analyses.
- Subjects
VASCULAR endothelial growth factor genetics; BLOOD sampling; SINGLE nucleotide polymorphisms; COLON cancer patients; FORMALDEHYDE; TISSUE fixation (Histology)
- Publication
International Journal of Molecular Sciences, 2014, Vol 15, Issue 8, p13333
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms150813333