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- Title
The Role of Nitric Oxide Synthase in an Early Phase Cd-Induced Acute Cytotoxicity in MCF-7 Cells.
- Authors
Zhong, Lingying; Wang, Lumei; Xu, Lurong; Liu, Qunlu; Jiang, Linlei; Zhi, Yuee; Lu, Wei; Zhou, Pei
- Abstract
Literature to date has confirmed that cadmium (Cd) can accomplish its toxic effects via the free radical-induced damage, but Cd itself cannot generate free radicals directly. Nitric oxide (NO) is a fundamental molecule that interplays with reactive oxygen species (ROS), which may be associated with the Cd-induced cytotoxicity. However, the role of nitric oxide synthase (NOS) in an early phase Cd-induced acute cytotoxicity and its interaction has not been studied. In this report, we provide data showing that CdCl (10 μM, 100 μM, 1 mM) could modulate NOS activity in terms of NO production which was first suppressed with the release of Ca and Zn, then induced with the transcriptional and translational activation of the three NOS isoforms in a possible feedback manner. The ROS level in cells was increased after CdCl exposure. By using the free radical scavenger N-acetyl- l-cysteine (LNAC) or the NOS activity inhibitor N-methyl- l-arginine (LNMMA), it was demonstrated that NOS played a critical role on the Cd-induced ROS generation. The Cd-induced cytotoxicity was associated with the NOS-mediated oxidative stress in MCF-7 cells.
- Subjects
REJUVENESCENCE (Botany); REACTIVE oxygen species; SULFUR amino acids; HABER-Weiss reaction; CYSTEINE
- Publication
Biological Trace Element Research, 2015, Vol 164, Issue 1, p130
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-014-0187-1