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- Title
Neuroglobin Involvement in the Course of Arsenic Toxicity in Rat Cerebellar Granule Neurons.
- Authors
Liu, Xiaona; Gao, Yanhui; Yao, Hongju; Zhou, Lingwang; Sun, Dianjun; Wang, Jing
- Abstract
Exposure to arsenic in drinking water results in a widespread environmental problem in the world, and the brain is a major target. Neuroglobin is a vertebrate heme protein regarded as playing neuroprotective role in hypoxia or oxidative stress. In this study, we investigated the toxic effects of sodium arsenite (NaAsO) on primary cultured rat cerebellar granule neurons (CGNs) and detected neuroglobin (Ngb) expression in rat CGNs exposed to NaAsO. Our results show that apoptosis was obviously induced by NaAsO treatment in rat CGNs by annexin V-fluorescein isothiocyanate assay. Intracellular reactive oxygen species generation increased significantly in the cells exposed to NaAsO, and the apoptotic effects could be partially reversed by antioxidant N-acetyl- l-cysteine. Ngb protein and mRNA expression were significantly downregulated in rat CGNs shortly after NaAsO exposure and then upregulated after a longer time of exposure. Furthermore, mRNA expression changed more than protein expression and the toxic effect of NaAsO on Ngb expression is dose dependent. Higher Ngb expression was also detected in rat cerebellum, but not in other parts (cerebrum, hippocampus, and midbrain) of the brain exposed to NaAsO for 16 weeks. Taken together, cytotoxic effects of NaAsO on rat CGNs is induced at least partly by oxidative stress and Ngb may influence the course of arsenic toxicity in rat CGNs and rat cerebellum.
- Subjects
GLOBIN; ARSENIC poisoning; LABORATORY rats; CEREBELLAR nuclei; NEURONS; OXIDATIVE stress; SODIUM arsenite
- Publication
Biological Trace Element Research, 2013, Vol 155, Issue 3, p439
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-013-9810-9