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- Title
Fluorescence Reporting of Binding Interactions of Target Molecules with Core-Shell-Type Cortisol-Imprinted Polymer Particles Using Environmentally Responsible Fluorescent- Labeled Cortisol.
- Authors
Murase, Nobuo; Taniguchi, Shin‐Ichi; Takano, Eri; Kitayama, Yukiya; Takeuchi, Toshifumi
- Abstract
Core-shell-type molecularly imprinted polymer particles (MIP-NPs) bearing specific binding cavities for cortisol in their shell layers by two-step emulsifier-free emulsion polymerizations are synthesized, where both the strategies of semicovalent molecular imprinting and noncovalent molecular imprinting are concurrently performed using methacryloyl cortisol and itaconic acid as a template molecule and a functional monomer, respectively. Newly designed fluorescent reporter molecules, dansyl-labeled cortisol, to investigate the binding capability of MIP-NPs toward cortisol by fluorescence measurements are developed. The binding rate constant (ka) and affinity constant (Ka) of MIP-NPs toward cortisol are successfully estimated by curve fitting for fluorescence spectral shift. The ka value of MIP-NPs is 1.8 times larger than that of reference MIP-NPs prepared using only methacryloyl cortisol without itaconic acid (semicovalent imprinting), indicating that the concurrent demonstration of both the semicovalent imprinting and noncovalent imprinting processes is effective for constructing high affinity binding cavities for cortisol.
- Subjects
PARTICLES; HYDROCORTISONE; EMULSION polymerization; MOLECULAR imprinting; ITACONIC acid; MOLECULES; MONOMERS
- Publication
Macromolecular Chemistry & Physics, 2015, Vol 216, Issue 13, p1396
- ISSN
1022-1352
- Publication type
Article
- DOI
10.1002/macp.201500065