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- Title
Cloning and expression of P450c17-I (17α-hydroxylase/17,20-lyase) in brain and ovary during gonad development in Cynoglossus semilaevis.
- Authors
Chen, Cai F.; Wen, Hai S.; Wang, Zhao P.; Feng He; Zhang, Jia R.; Chen, Xiao Y.; Jin, Guo X.; Bao Shi; Dan Shi; Yang, Yan P.; Li, Ji F.; Qi, Bao X.; Na Li
- Abstract
Cytochrome P450c17 (CYP17, 17a-hydroxylase/17,20-lyase) is a critical enzyme in the production of androgens and estrogens in vertebrates. A 2,469 bp full length cDNA of P450c17-I ( CYP17A1) has been isolated from the ovary of half-smooth tongue sole, Cynoglossus semilaevis which encodes 509 amino acids. Additionally, a relatively shorter cDNA (1,742 bp), a likely result of polyadenylation, was also found. The putative P450c17-I enzyme shares high sequence identity with that of the fathead minnow (73%), zebrafish (71%), the Japanese eel (70%), catfish (70%), tilapia (79%), three-spined stickleback (81%), medaka (79%), dogfish (60%), chicken (65%), rat (47%), and human (49%). Semi-quantitative RT-PCR analysis of spatial expression showed the enzyme was predominantly expressed in the ovaries and the brain. P450c17-I was also detected in the stomach, intestine, gill, spleen, kidney, and head kidney, albeit weakly. Further examination of temporal expression pattern of P450c17-I in ovary and brain revealed developmental stage-dependency. In addition to this our data on T and E2 levels further endorse the critical role of P450c17-I during shift in steroidogenesis. Based on the present study we indicate an important role for P450c17-I during ovarian development. However, further studies are needed at transcriptional regulation level for deeper insights into the physiological functions of P450c17-I.
- Subjects
CYNOGLOSSIDAE; ENZYMES; SEX hormones; IMINO acids; AMINO acids
- Publication
Fish Physiology & Biochemistry, 2010, Vol 36, Issue 4, p1001
- ISSN
0920-1742
- Publication type
Article
- DOI
10.1007/s10695-009-9378-7