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- Title
Modulation of heat shock protein expression and cytokine levels in MCF-7 cells through photodynamic therapy.
- Authors
dos Santos, Mariela Inês Batista; Godoi, Bruno Henrique; Da Silva, Newton Soares; Oliveira, Luciane Dias; de Paula Ramos, Lucas; Cintra, Ricardo Cesar; Pacheco-Soares, Cristina
- Abstract
In this study, we assess the impact of photodynamic therapy (PDT) using aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability and cellular stress responses of MCF-7 breast cancer cells. Specifically, we investigate changes in cell viability, cytokine production, and the expression of stress-related genes. Experimental groups included control cells, those treated with AlPcS4 only, light-emitting diode (LED) only, and combined PDT. To evaluate these effects on cell viability, cytokine production, and the expression of stress-related genes, techniques such as 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, enzyme-linked immunosorbent assays (ELISA), and real-time quantitative PCR (RT‒qPCR) were employed. Our findings reveal how PDT with AlPcS4 modulates mitochondrial activity and cytokine responses, shedding light on the cellular pathways essential for cell survival and stress adaptation. This work enhances our understanding of PDT's therapeutic potential and mechanisms in treating breast cancer.
- Subjects
HEAT shock proteins; PHOTODYNAMIC therapy; PROTEIN expression; CYTOKINES; ENZYME-linked immunosorbent assay; CELL survival; METASTATIC breast cancer
- Publication
Lasers in Medical Science, 2024, Vol 39, Issue 1, p1
- ISSN
0268-8921
- Publication type
Article
- DOI
10.1007/s10103-024-04092-1