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- Title
Specific transporter for iron(III)-phytosiderophore complex involved in iron uptake by barley roots.
- Authors
Murata, Yoshiko; Harada, Emiko; Sugase, Kenji; Namba, Kosuke; Horikawa, Manabu; Jian Feng Ma; Yamaji, Naoki; Ueno, Daisei; Nomoto, Kyosuke; Iwashita, Takashi; Kusumoto, Shoichi
- Abstract
Iron (Fe) is an essential element for plant growth. Gramineous plants have generally developed a distinct strategy to efficiently acquire insoluble Fe, which is characterized by the synthesis and secretion of an Fe-chelating substance, phytosiderophore (PS) such as mugineic acid (MA), and by a specific uptake system for Fe(III)-PS complexes. In a previous study, we identified a gene specifically encoding an Fe(III)-PS transporter (HvYS1) in barley. This gene as well as the encoded protein is specifically expressed in the epidermal cells of the roots, and gene expression is greatly enhanced under Fe-deficient conditions. The localization and substrate specificity of HvYS1 indicate that it is a Fe(III)-PS specific transporter in barley roots. In contrast, ZmYS1, which has been reported as an Fe-PS transporter from maize, possesses broad substrate specificity despite a high homology with HvYS1. By assessing the transport activity of a series of HvYS1-ZmYS1 chimeras, we revealed that the outer membrane loop between the 6th and 7th transmembrane regions is essential for the substrate specificity. We also achieved an efficient short-step synthesis of MA and 2'-deoxymugineic acid (DMA). Our new synthetic method enabled us to use them in a large quantity for biological studies.
- Subjects
BARLEY; EFFECT of iron on plants; IRON chelates; GENE expression; ROOT development; CORN; PLANT growing media
- Publication
Pure & Applied Chemistry, 2008, Vol 80, Issue 12, p2689
- ISSN
0033-4545
- Publication type
Article
- DOI
10.1351/pac200880122689