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- Title
Ameliorative Effect of Citropten Isolated from Citrus aurantifolia Peel Extract as a Modulator of T Cell and Intestinal Epithelial Cell Activity in DSS-Induced Colitis.
- Authors
Lee, Hyun-Su; Kim, Eun-Nam; Jeong, Gil-Saeng
- Abstract
Citropten is a coumarin that is mainly found in fruits of Rutaceae trees, but its anti-inflammatory activities in colitis is still unknown. In this study, we investigated its attenuating effect of citropten isolated from Citrus aurantifolia extract on DSS-induced colitis through the modulation of the activity of T cells and intestinal epithelial cells. We found that pre-treatment with citropten downregulates the activity of T cells and intestinal epithelial cells without a negative effect on the viability of Jurkat and HT-29 cells. The results from the Western blot analysis revealed that pre-treatment with citropten reduces the NFκB and MAPK signaling pathway in activated T cells and intestinal epithelial cells. We elucidated that the oral administration of citropten alleviates the colonic inflammation and activity of effector T cells in DSS-induced colitis by measuring changes in body weight, histological scoring from H&E-stained sections, mRNA levels of pro-inflammatory cytokines and the phosphorylation level of the MAPK signaling pathway.
- Subjects
EPITHELIAL cells; T cells; COLITIS; WESTERN immunoblotting; CITRUS
- Publication
Molecules, 2022, Vol 27, Issue 14, pN.PAG
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27144633