We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Immunobiological peculiarities of cholesteatoma in children: quantification of epithelial proliferation by MIB1.
- Authors
Bujía, Jesús; Holly, Anja; Antolí-Candela, Francisco; Tapia, Mario Guzman; Kastenbauer, Ernst; Bujía, J; Holly, A; Antolí-Candela, F; Tapia, M G; Kastenbauer, E
- Abstract
Cholesteatoma in children is characterized by a more extensive and rapid growth in the middle ear and mastoid cavities. The growth characteristics of the cholesteatoma in 20 children were studied using the monoclonal antibody MIB 1, which recognizes a nuclear antigen expressed by cells in the G1, S, and G2/M phases. Specimens of normal adult auditory meatal skin (n = 15) and adult cholesteatoma (n = 15) served as controls. The tissue specimens were prepared for immunohistochemical examination using the alkaline phosphatase-antialkaline phosphatase method and an automatic image analyzer. Specimens of normal skin revealed an average MIB 1 score of 9.2 +/- 3.10%. Child and adult cholesteatomas showed higher values. The average MIB 1 score was higher in child cholesteatoma (42 +/- 9.4%) than in adult cholesteatoma (28.2 +/- 6%). This difference was statistically significant (P<.01). Our results confirm a significant increase of the proliferative rate of cholesteatoma keratinocytes in children, giving an explanation for the more aggressive clinical behavior observed in these patients.
- Publication
Laryngoscope, 1996, Vol 106, Issue 7, p865
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1097/00005537-199607000-00015