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- Title
Development of a Method for Isolation of Mature Cardiomyocytes from Human Heart Biopsy Specimens.
- Authors
Kovalenko, S. G.; Frolova, Sh. R.; Kramkova, V. K.; Berezovskii, A. K.; Popov, M. A.; Shumakov, D. V.; Zybin, D. I.; Agafonov, E. G.; Dontsov, V. V.; Agladze, K. I.
- Abstract
To increase the yield of living cells and their survival, studies were carried out to optimize the method for isolating cardiomyocytes from biopsy specimens excised from the right atrial appendages. It was found that creatine, blebbistatin, and taurine are necessary components of the buffer solution during cardiomyocyte isolation, and that composition of the solutions is a more important factor than their oxygenation.
- Subjects
HEART; BIOPSY; BUFFER solutions; CELL survival; TAURINE; CREATINE
- Publication
Bulletin of Experimental Biology & Medicine, 2023, Vol 175, Issue 4, p585
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-023-05907-x