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- Title
Cannabis sativa L. photoautotrophic micropropagation: a powerful tool for industrial scale in vitro propagation.
- Authors
Zarei, Adel; Behdarvandi, Behrang; Tavakouli Dinani, Elham; Maccarone, Jennifer
- Abstract
Global demands for an in vitro culture of cannabis have never been more sought after as countries shift their paradigm towards legalization. Cannabis conventional (photomixotrophic) micropropagation has not been suitable enough for large-scale propagation due to a high degree of plant hyperhydricity, low growth rate, poor rooting, and acclimation efficiency. In the present study, cannabis photoautotrophic micropropagation method is introduced with the purpose of overcoming the difficulties that conventional micropropagation entails when conducted at a large scale. The roles of rockwool medium pH and moisture content, cutting length, basal wounding methods, light intensity, and culture vessel gas exchange capacity were assessed with the intention of increasing productivity of micropropagation method. The results showed 300 mL per vessel of fertilizer solution containing 5-mM MES buffer stabilized medium pH and increased rooting success. Both 5- and 7-cm cutting lengths significantly increased the percent of rooted plants compared to 3-cm cutting length. However, the basal wounding methods did not significantly improve or impede the rooting success. The highest rooting success was also obtained with 150 μmol m−2 s−1 of photosynthetic photon flux density compared to 50 and 100 μmol m−2 s−1. Increasing gas exchange rates either using more permeable vessels or aeration practices significantly improved the rooting success. Overall, more than 90% of cannabis plantlets grown in photoautotrophic micropropagation are rooted in 2 wk of culture followed by 4 d ex vitro acclimation period, which was remarkably shorter than any other available method in cannabis micropropagation. This study not only optimizes a method for cannabis photoautotrophic micropropagation using passive ventilation for the first time but also scales up in vitro clonal propagation for in vitro commercial production.
- Subjects
CANNABIS (Genus); ACTINIC flux; PHOTON flux; LIGHT intensity; ACCLIMATIZATION
- Publication
In Vitro Cellular & Developmental Biology Plant, 2021, Vol 57, Issue 6, p932
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-021-10167-3