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- Title
Insulin-producing cells from human adipose tissue-derived mesenchymal stem cells detected by atomic force microscope.
- Authors
Shi, Qiping; Luo, Simin; Jin, Hua; Cai, Jiye; Jia, Haiying; Feng, Lie; Lu, Xiaohua
- Abstract
We successfully differentiated human adipose tissue-derived mesenchymal stem cells (haMSCs) into insulin-producing cells (IPCs) in vitro and did not use any insulin which might be absorbed by cells during in vitro culture. Expression of insulin gene was massively increased by 28,000-fold at day 12 compared with haMSCs ( P < 0.05). IPCs could secrete insulin after glucose was stimulated. The higher the concentration of glucose, the more production of insulin was noted. We reported AFM images of IPCs for the first time. AFM images showed that the sizes of cells were similar to each other, and all IPC surface had a porous structure in the cytoplasm area. In sugar-free group, the size of holes was similar (diameter, 1,086.98 ± 156.70 nm; depth, 185.22 ± 52.14 nm). In higher sugar-stimulated group, there were more holes with bigger diameter and smaller depth. (diameter, 3,183.65 ± 2,229.18 nm; depth 109.42 ± 56.26 nm, P < 0.05). We found that the hole diameter and depth could change with the concentration of glucose in media. Concurrently, laser scanning confocal microscopy images indicated that cortical actin network beneath plasma membrane in IPCs was dense and continuous. After glucose stimulation, we found the actin web depolymerized and became discontinuous in IPCs. We speculated that diameter augmentation of holes located in the cytoplasm area in IPCs was one manifestation of excytosis increase.
- Subjects
ADIPOSE tissues; MESENCHYMAL stem cells; INSULIN biotechnology; ATOMIC force microscopy; GLUCOSE synthesis
- Publication
Applied Microbiology & Biotechnology, 2012, Vol 94, Issue 2, p479
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-012-3904-8