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- Title
Contribution of an adenine to guanine single nucleotide polymorphism of the matrix metalloproteinase-13 ( MMP- 13) −77 promoter region to the production of anticyclic citrullinated peptide antibodies in patients with HLA-DRB1*shared epitope-negative rheumatoid arthritis
- Authors
Iwamoto, Naoki; Kawakami, Atsushi; Arima, Kazuhiko; Tamai, Mami; Nakamura, Hideki; Kawashiri, Shin-ya; Kita, Junko; Okada, Akitomo; Koga, Tomohiro; Kamachi, Makoto; Yamasaki, Satoshi; Ichinose, Kunihiro; Ida, Hiroaki; Origuchi, Tomoki; Eguchi, Katsumi
- Abstract
We examined whether matrix metalloproteinase-13 (MMP-13) contributes to disease susceptibility or severity of rheumatoid arthritis (RA). Eighty-seven patients with RA whose disease duration was <2 years and 71 healthy controls were enrolled in the study. Adenine (A) to guanine (G) single nucleotide polymorphism (SNP) of the −77 MMP- 13 promoter region in RA and healthy controls was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Human leukocyte antigen (HLA)-DRB1 genotyping was also performed using the same populations. Anticyclic citrullinated peptide (anti-CCP) antibodies from RA patients at entry were studied, and their relationships were examined. The genotype and allele frequency of SNP of MMP- 13 at −77 did not differ between RA patients and healthy controls. We focused on the RA patients who were negative for HLA-DRB1*shared epitope (SE) alleles and found that the seropositivity of anti-CCP antibodies with a titer >25 U/ml was high in the A/A genotype compared with the G/G genotype. The same characteristic was also found in HLA-DRB1*0405 allele-negative patients. Our data suggest that SNP of the −77 MMP- 13 promoter region acts as a surrogate marker of anti-CCP antibody production in HLA-DRB1*SE allele-negative RA patients, which may reflect RA severity.
- Subjects
ADENINE; NUCLEOTIDE sequence; METALLOPROTEINASES; HLA histocompatibility antigens; GENETIC polymorphisms; RHEUMATOID arthritis; DISEASE susceptibility; GENETIC markers
- Publication
Modern Rheumatology, 2011, Vol 21, Issue 3, p240
- ISSN
1439-7595
- Publication type
Article
- DOI
10.3109/s10165-010-0375-6