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- Title
ANTI-ADHESIVE ACTIVITY OF PHENAZINE PRODUCED BY P. AERUGINOSA ISOLATED FROM RHIZOSPHERE PLANT ROOT FROM IRAQI SOIL AGAINST SOME WOUND AND BURN INFECTION BACTERIA.
- Authors
Ali, Ali H.; Mussa, Amel H.
- Abstract
The study including extraction and purification of phenazine produce from P. aeruginosa (PS 33) isolated from rhizospheric plantand studying its effect on wound & burn pathogenic bacteria isolated from locally hospitals in Baghdad, Iraq. The isolates involving Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Serratia marcescens, Streptococcus pyogens and Staphylococcus aureus. PS33 was culturing on King’s B broth and extracted crude phenazine with benzene, purified it with TLC (Thin layer chromatography), RF - value obtained was 0.7. Then exposure to HPLC (High performance liquid chromatography) to detected the phenazine on wave length 248 nm. Single peak appeared on retention time 5.1, which related to phenazine. Antibacterial of crude and purified phenazine against Multi drug resistance (MDR) isolate of wound & burn infections. The results appeared that crude phenazine had greater activity than purifiedand the Staphylococcus aureus was more sensitive to crude phenazine. Antiadhesive activity of phenazine was distinguished and the results revealed that phenazine showed good activity as antiadhesive against pathogenic bacteria highly activity of phenazine against wound & burn bacterial isolates were reaching to 48.6% for Acinetobacter baumanii.
- Subjects
PHENAZINE; STAPHYLOCOCCUS aureus; MULTIDRUG resistance in bacteria; ACINETOBACTER baumannii; PATHOGENIC bacteria
- Publication
Biochemical & Cellular Archives, 2020, Vol 20, Issue 1, p1073
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2020.20.1.1073