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- Title
Basophil activation test is reliable for inhalant allergens in pediatric patients.
- Authors
Ogulur, Ismail; Kiykim, Ayca; Baris, Safa; Ozen, Ahmet; Onat, Ezgi Gizem; Bakir, Mustafa; Karakoc Aydiner, Elif; Barlan, Isil
- Abstract
Background: Flow cytometric quantification of in vitro basophil activation can be quite performant and reliable tool to measure IgE-dependent allergen-specific responses in allergic patients. The aim of this study was to determine sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) of BAT in our patients sensitized to house dust mite (HDM) and grass pollen with allergic rhinitis and/ or asthma. Methods: Forty-seven children (35 M/ 12 F, 11±3 years) sensitized to house dust mite and grass pollen with a clinical history of allergic rhinitis and/or asthma were enrolled. To confirm sensitization, a positive skin prick test (SPT) or elevatated specific IgE to Dermatophagoides farina (DF), Dermatophagoides pteronyssinus (DP) and grass pollen were used. The non-allergic healthy control group (n=18) had no history of allergy with negative skin prick tests to the common allergens tested. The BASOTEST kit (Glycotope Biotechnology GmbH, Heidelberg, Germany) was used to carry out BAT according to the manufacturer's instruction. Basophil activation was determined by flow cytometry upon double staining with anti- IgE/anti-CD63 mAb. The upregulation of the activation marker CD63 was calculated by the percentage of the CD63-positive cells compared with the total identified basophilic cells. Results: Receiver operating characteristics (ROC) curve analyses yielded a cut-off level for positive response as 15% for BAT with HDM and grass pollen when patients and healthy controls were all evaluated. For HDM, the sensitivity, specificity, PPV, and NPV of the basophil activation were 90%, 73%, 0.70 and 0.91, respectively. The analysis of concordance of being either allergic or healthy in comparison to BAT results for HDM revealed a substantial concordance (κ index= 0.61, p<0.001). For grass pollen, BAT attained a sensitivity, specificity, PPV, and NPV of 96%, 93%, 0.98 and 0.88, respectively. The analysis of concordance of being either allergic or healthy in comparison to BAT results for grass pollen revealed an almost perfect concordance (κ index= 0.87, p<0.001). Conclusion: Our results confirmed BAT as reliable and easy method to detect basophil activation in response to grass pollen and HDM in pediatric inhalant allergic patients. The obtained specificity, sensitivity, PPV and NPV values for these allergens were also found to be comparable with the levels in the literature.
- Subjects
BASOPHILS; PEDIATRICS; FLOW cytometry; HOUSE dust mites; ALLERGIC rhinitis; PATIENTS
- Publication
Turkish Journal of Immunology, 2016, Vol 4, p65
- ISSN
1301-109X
- Publication type
Article