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- Title
Expression pattern of sonic hedgehog and effect of topical mitomycin C on its expression in human ocular surface neoplasms.
- Authors
Kyoko Fujita; Takeshi Miyamoto; Yuka Okada; Nobuyuki Ishikawa; Shizuya Saika
- Abstract
Abstract Purpose To examine if the cells of human ocular surface neoplasms express sonic hedgehog (Shh) and the effects of topical mitomycin C on its expression. Methods Conjunctival tissues obtained from two normal subjects, two patients with squamous cell carcinoma of the ocular surface (conjunctiva), and one patient with ocular epithelial dysplasia were used in this study. Histological sections were processed for light microscopic immunohistochemical analysis for Shh. Results Faint immunoreactivity for Shh was detected in basal epithelial cells of limbus, bulbar, and palpebral conjunctival epithelial cells. On the other hand, squamous cell carcinoma cells markedly expressed Shh with positive staining for Patched 1(Ptc), the cell surface receptor of Shh. Similar marked expression of Shh was detected in the patient with ocular epithelial dysplasia, and this Shh expression was almost eliminated following topical mitomycin C treatment. A cell culture experiment was conducted to examine the effect of mitomycin C on Shh expression in a cultured squamous cell carcinoma cell line. Conclusions Conjunctival epithelium constitutively expresses a low level of Shh, and its expression increases during malignant conversion of epithelial cells. Reduction of Shh expression might be involved in the therapeutic efficacy of topical mitomycin C for ocular surface epithelial neoplasms.
- Subjects
TUMORS; MITOMYCIN C; CANCER patients; IMMUNOSUPPRESSIVE agents; TISSUES; SQUAMOUS cell carcinoma; EPITHELIAL cells
- Publication
Japanese Journal of Ophthalmology, 2008, Vol 52, Issue 3, p190
- ISSN
0021-5155
- Publication type
Article
- DOI
10.1007/s10384-008-0527-9