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- Title
In vitro Studies of the Utilization of Industrially Important Substrates by Lactobacillus sp. AAF-1 Isolated from Coconut Water.
- Authors
Siddiqui, Ayesha; Aman, Ayisha; Fatima, Syeda Areej; Riaz, Aliya; Zohra, Rashida Rahmat; Naheed, Suad
- Abstract
Coconut water (Cocos nucifera L.) possesses natural hydrating qualities, functional health properties and various nutritional benefits. Lactobacillus species AAF-1 has been isolated and purified from coconut water. The isolated culture was screened for the production of extracellular secreted enzymes having industrial value. Growth of the Lactobacillus sp. AAF-1 on MRS agar containing respective substrates showed that the Lactobacillus sp. AAF-1 is the potential producer of amylase, protease, cellulase and beta galactosidase. Further cell free filtrates of the Lactobacillus sp. AAF-1 showed large zone of hydrolysis of starch, gelatin, cellulose and lactose that reflected the extracellular production of the above enzymes in culture medium at 50 °C. It has been observed that activities after 72 h of incubation in cell free filtrates of protease, amylase, cellulase and beta galactosidase as the zone of respective substrate hydrolysis measured as 10, 8, 6 and 10 mm respectively. Thus, Lactobacillus sp. AAF-1 could be the potential producer of the enzymes having industrial value.
- Subjects
COCONUT water; GALACTOSIDASES; LACTOBACILLUS; EXTRACELLULAR enzymes; IN vitro studies; COCONUT palm
- Publication
Pakistan Journal of Scientific & Industrial Research Series B: Biological Sciences, 2022, Vol 65, Issue 1, p62
- ISSN
2221-6421
- Publication type
Article
- DOI
10.52763/pjsir.biol.sci.65.1.2022.62.66