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- Title
Morphology and characteristic of WJ-MSC expanded with different growth mediums.
- Authors
Bin Hassan, Muhammad Najib Fathi; Yazid, Muhammad Dain; Mohd Yunus, Mohd Heikal Bin; Idrus, Ruszymah Bt Hj; Lokanathan, Yogeswaran; Min Hwei Ng; Ng See Nguan; Jia Xian Law
- Abstract
Introduction: Mesenchymal stem cells (MSCs) derived from Wharton's jelly (WJ-MSCs) have many advantages, such as being easy to obtain, having immunosuppressive properties, multi-lineage differentiation potential and no ethical issues. Due to these advantages, WJ-MSCs are a promising candidate for therapeutic applications. To date, many types of mediums have been developed to expand WJ-MSCs. This study aims to compare the effects of different mediums on the proliferation, safety, and quality of WJ-MSCs for potential large-scale cell expansion. Methods: A total of 5 different growth mediums, i.e., DMEM-LG + 10% FBS, DMEM-LG + 10% HPL, StemMacs, MSC-brew GMP medium and Mesencult-HPL, were tested. The cells' growth kinetic was analyzed by determining the cell viability, cell yield, and population doubling time. WJ-MSCs cultured with selected growth mediums were characterised as being cultured through cell surface CD marker, tri-lineage differentiation potential, immunosuppression assay and cell cycle assay. Results: WJ-MSCs cultured with DMEM-LG + 10% HPL, StemMacs and MSC-brew GMP medium were smaller in size than those expanded with the other mediums. Cell yield was significantly higher and population doubling time was significantly shorter when WJ-MSCs were cultured with DMEM-LG + 10% HPL, StemMacs and MSC-brew GMP medium. Thus, only these three mediums were proceeded to WJ-MSCs characterization. The DMEM-LG + 10% HPL gave higher cell yield at lower seeding density than those expanded with StemMacs and MSC-brew GMP medium. WJ-MSCs cultured with all three mediums expressed the MSCs surface markers, able to suppress PBMC proliferation, capable of differentiating into adipogenic, chondrogenic and osteogenic lineages, and most of the cells were in G1 phase compared to S phase and G2 phase. Conclusion: DMEM-LG+10% HPL is the best medium for the expansion of WJ-MSCs as it can provide higher cell yield at lower passage without compromising the cell proliferation, quality and safety.
- Subjects
MESENCHYMAL stem cells; CELL growth; CELL cycle; MORPHOLOGY; CELL culture
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p8
- ISSN
1675-8544
- Publication type
Abstract